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alexa fluor 488 donkey anti guinea pig igg h l  (Jackson Immuno)


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    Structured Review

    Jackson Immuno alexa fluor 488 donkey anti guinea pig igg h l
    Alexa Fluor 488 Donkey Anti Guinea Pig Igg H L, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+rabbit+alexa+fluor+405/DyLight+405+AffiniPure+Donkey+Anti-Rabbit+IgG/pmc12928769-148-16-26
    Average 95 stars, based on 237 article reviews
    alexa fluor 488 donkey anti guinea pig igg h l - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Network state-dependent inhibition of identified hippocampal CA3 axo-axonic cells in vivo
    Article Snippet: The following secondary antibodies were used: donkey anti-rabbit Alexa Fluor 405 (1:250, Invitrogen; {"type":"entrez-nucleotide","attrs":{"text":"A31556","term_id":"1567156","term_text":"A31556"}} A31556 lot 55310A); donkey anti-mouse, anti-goat or anti–guinea pig DyLight405 (1:250, Jackson ImmunoResearch Laboratories; 715-475-151 lot 98883, 705-475-147 lot 96706, 706-475-148 lot 99779, respectively); donkey anti-rabbit or anti-goat Alexa Fluor 488 (1:1,000, Invitrogen; {"type":"entrez-nucleotide","attrs":{"text":"A21206","term_id":"583478","term_text":"A21206"}} A21206 lot 556439, {"type":"entrez-nucleotide","attrs":{"text":"A11055","term_id":"490909","term_text":"A11055"}} A11055 lot 49732A, respectively); donkey anti-rabbit, anti-goat, anti-sheep, anti-mouse or anti–guinea pig Cy3 (1:400, Jackson; 711-165-152 lot 98888, 705-165-147 lot 81185, 713-165-147 lot 87933, 715-165-151 lot 94510, 706-165-148 lot 86928, respectively); donkey anti-rabbit, anti-goat, anti-sheep, anti-mouse or anti–guinea pig Cy5 (1:250, Jackson; 711-175-152 lot 80443, 705-175-147 lot 72031, 713-175-147 lot 72156, 715-175-151 lot 88814, 706-175-148 lot 72155, respectively); donkey anti-rabbit, anti-goat or anti-mouse DyLight 649 (1:250, Jackson; 711-495-152 lot 98016, 705-495-147 lot 97534, 715-495-151 lot 94507, respectively), donkey anti-sheep or anti–guinea pig Alexa Fluor 647 (1:250, Jackson; 713-605-147 lot 102770, 706-605-148 lot 100905, respectively); donkey anti–guinea pig HRP (1:100, Jackson); and swine anti-rabbit HRP (1:100, Dako, Glostrup, Denmark; P0217).

    Incubation:

    Article Title: Polysialic Acid Acute Depletion Induces Structural Plasticity in Interneurons and Impairs the Excitation/Inhibition Balance in Medial Prefrontal Cortex Organotypic Cultures
    Article Snippet: Slices were incubated overnight at room temperature with the following cocktails of primary antibodies: (1) Chicken anti-GFP (Chemicon-Millipore, 1:1000) and mouse IgM anti-PSA-NCAM; (2) Chicken anti-GFP, mouse IgG2b anti-calcium/calmodulin-dependent kinase II-α (CaMKII-α; Abcam, 1:500) and rabbit anti-vesicular GABA transporter (VGAT; Synaptic Systems, 1:1000); (3) Chicken anti-GFP, mouse IgG anti-CaMKII-α and rabbit anti-synaptophysin (SYN; Chemicon-Millipore, 1:1000); (4) Chicken anti-GFP, mouse IgG anti-CaMKII-α and rabbit anti-parvalbumin (PV; Swant, 1:2000); (5) Chicken anti-GFP, mouse IgG anti-CaMKII-α and guinea pig anti-vesicular glutamate transporter 1 (VGLUT1; Chemicon-Millipore, 1:2000); (6) Mouse IgM anti-PSA-NCAM, mouse IgG2b anti-CaMKII-α and rabbit anti-calretinin (CR; Swant, 1:1000); and (7) Mouse IgM anti-PSA-NCAM, guinea pig anti-PV (Synaptic Systems, 1:4000) and rabbit anti-calbindin (CB; Swant, 1:1000). .. After washing, sections were incubated for 1 h with five different cocktails of fluorescent secondary antibodies: (1) Donkey anti-chicken Dylight 488 (Jackson Immunoresearch, 1:400) and donkey anti-mouse IgM Dylight 555 (Jackson Immunoresearch, 1:400); (2) Donkey anti-chicken Dylight 488, mouse IgG Dylight 647 (Jackson Immunoresearch, 1:400) and donkey anti-rabbit Dylight 555 (Jackson Immunoresearch, 1:400); (3) Donkey anti-chicken Dylight 488, donkey anti-mouse IgG Dylight 647 and goat anti-guinea pig Dylight 549 (Jackson Immunoresearch, 1:400); (4) Donkey anti-mouse IgG2b Dylight 405 (Jackson Immunoresearch, 1:400), donkey anti-mouse IgM Dylight 549 (Jackson Immunoresearch, 1:400) and donkey anti-rabbit Dylight 649 (Jackson Immunoresearch, 1:400); and (5) Donkey anti-mouse IgM Dylight 549, goat anti-guinea pig Dylight 649 (Jackson Immunoresearch, 1:400) and donkey anti-rabbit Alexa Fluor 405 (Jackson Immunoresearch, 1:400). .. Finally, sections were washed, mounted on slides and coverslipped using DakoCytomation fluorescent mounting medium (Dako North America Inc., Carpinteria, CA, USA).

    Article Title: In vivo expression of VCAM1 precedes nephron loss following kidney tubular necrosis
    Article Snippet: Kidney tissue stained against VCAM1 was blocked in 1% bovine serum albumin (BSA)/2% Sea Block/0.1% Triton X-100/PBS for 1 hour and then incubated in rabbit anti-VCAM1 (Abcam, AB0134047; 1:200) for 72 hours at 4°C. .. After washing in PBS (3× for 5 min), donkey anti-rabbit Alexa Fluor 405 (Jackson ImmunoResearch, 711-175-152; 1:500) secondary antibody was incubated overnight at 4°C. .. For PDGFRβ, the kidney tissue was blocked with 1% BSA + 2% Sea Block in PBS for 1 hour and incubated in rabbit anti-PDGFRβ (Abcam, ab32570; 1:200) overnight at 4°C.

    Blocking Assay:

    Article Title: Transcriptional Regulation of Microglial Metabolic and Activation States by P2RY12.
    Article Snippet: Microglia are the resident immune cells of the CNS.. Under homeostatic conditions, microglia play critical roles in orchestrating synaptic pruning, debris clearance, and dead cell removal.. In disease, they are powerful mediators of neuroinflammation, as they rapidly respond to injury or infection within the CNS by altering their morphology, proliferating, and releasing cytokines and other signaling molecules.



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    Antibodies used in this study

    Journal: mBio

    Article Title: Inhibition and evasion of neutrophil microbicidal responses by Legionella longbeachae

    doi: 10.1128/mbio.03274-24

    Figure Lengend Snippet: Antibodies used in this study

    Article Snippet: After primary labeling, cells were labeled with donkey anti-rabbit Alexa Fluor 405 (1:250 dilution, Invitrogen #A48257), donkey anti-mouse Alexa Fluor 488 (1:2,000 dilution, Invitrogen #A32766), donkey anti-goat Alexa Fluor 555 (1:2,000 dilution, Invitrogen #A21432), and donkey anti-chicken Alexa Fluor 647 (1:2,000 dilution, Invitrogen #A78952).

    Techniques: